Producción de biofilm a partir de Komagataeibacter xylinus, utilizando diferentes residuos agroindustriales

Abstract

Bacterial biofilms are being an important field of study, due to their wide use in different fields. However, due to its high production costs, its use is limited. For this reason, this work aimed to obtain biofilms using different agroindustrial wastes (sugarcane bagasse, rice bran and cocoa husk), using an H-S medium and minimum medium in static and agitated culture. Demonstrating that agroindustrial wastes contributes to the medium the necessary carbon and nitrogen source, so that the bacterium Komagataeibacter xylinus can develop and generate the biofilms. The biofilms obtained from the cocoa husk showed the best yield in the H-S medium with 17,8338 and 22,8312 percent for the static and agitation culture respectively, while in the minimum medium a yield of 23,3169 and 16,1095 percent was obtained for the static and agitation culture correspondingly; it even showed that there is no statistically significant difference with the standard (glucose). Also, an impregnation with silver and zinc particles was made to the biofilms produced from the cocoa husk. Furthermore, by X-ray diffraction analysis (XRD), it was possible to determine that the biofilms obtained corresponded to Cellulose I, cellulose Iα being present in a higher proportion. The scanning electron microscopy (SEM) helped to observe the structure of the biofilms and by means of a Mapping the presence of the Ag and Zn nanoparticles impregnated in the structure of the biofilm was verified.