Identificación de hembras caninas domésticas en estro mediante la observación de cristalización de la saliva como método diagnóstico complementario comparado con citología vaginal

Abstract

The research work entitled, "Identification of estrus in domestic canine females, by observing the crystallization of saliva as a complementary diagnostic method compared to vaginal cytology", was carried out with the purpose of providing an alternative solution to the problem of estrous determination. in canine females, the method put to the test was the crystallization of saliva compared to vaginal cytology, the study was carried out in the facilities of the Hospital Clínico Veterinario Chile and the Animal Life Veterinary Clinic located in the north central sector of the city of Ambato, in which samples of vaginal mucosa and saliva were obtained for analysis by smear and direct observation under a microscope respectively, the two samples were analyzed at the same time and 2 additional shots were taken with an interval of 1 day between each obtaining, this was obtained of 30 canine female patients who presented sign proestrus and were within normal physiological parameters. To evaluate the vaginal mucous sample obtained by means of a vaginal swab, a slide smear was performed, using Wright stain as a dye, while for the saliva sample, which was obtained directly from the patient's oral cavity, the sample was fixed to ambient temperature for 60 seconds, and precedes its observation under the microscope. We used the statistical analysis of t through which it was possible to compare if there is a difference between the two methods used, the statistical test of t performed for the first sample, determines that there is no significant difference between the two methods therefore the crystallization method of saliva is feasible in this stage, for sample number 2 performed on the 3rd day a similar result is obtained with a statistical value of t of 1.43 that is within the range, demonstrating that the method evaluated remains reliable, while for the sample number 3 performed on the 5th day, a statistical value of t -2.79 was obtained that remains outside the range, resulting in a difference between both methods, with the results obtained in the 3 samples, 2 similar results are obtained between both methods and 1 unequal result, for which 66.6% reliability would be assigned to the estrus determination method by observing the saliva istalization. In conclusion, it was determined that the method of crystallization of saliva is considered feasible for the approximation towards the determination of estrus in domestic canine females taking into account that the studied method shows a slight difference in the last sample obtained.