Evaluación de hojas de tres especies de Musas spp. con adición de Saccharomyces cerevisiae y enzimas fibrolíticas exógenas sobre la función ruminal in vitro.

Abstract

The aim of this research was to evaluate the effect of the addition of Saccharomyces cerevisiae and exogenous fibrolytic enzymes in forages of Musa spp. on in vitro rumen function. The work was carried out in the experimental farm Querochaca of the UTA. The research was conducted under a completely randomized design with a 3 x 4 factorial arrangement. The data was analyzed according to the model used. The evaluated forages were added additives: S. cerevisiae and fibrolytic enzymes. To conform the following treatments: TO: M. acuminata without additives, TOE: M. acuminata with enzymes (0.002 mg/kg), TOS: M. acuminata with S. cerevisiae (0.002 mg/kg), TOES: M. acuminata with enzymes (0.002 mg/kg) and S. cerevisiae (0.002 mg/kg). TP: M. balbisiana without additives, TPE: M. balbisiana with enzymes (0.002 mg/kg), TPS: M. balbisiana with S. cerevisiae (0.002 mg/kg), TPES: M. balbisiana with enzymes (0.002 mg/kg) and S. cerevisiae (0.002 mg/kg) and TG: M. paradisiaca without additives, TGE: M. paradisiaca with enzymes (0.002 mg/kg), TGS: M. paradisiaca with S. cerevisiae (0.002 mg/kg), TGES: M. paradisiaca with enzymes (0.002 mg/kg) and S. cerevisiae (0.002 mg/kg). The production of total gas, CH4 and CO2, pH and digestibility and volatile fatty acids (VFAs) were determined. The total gas production was lower in the treatment TPE (265.5 mLgas/0.5g Fermented DM) compared to other treatments (P<.0001). The lowest (P=0.0001) CH4 production was observed in the treatment TO (36.3 mLCH4/0.5g Fermented DM), with respect to CO2 production was lower in the treatment TOS (71.3 mLCO2/0.5g Fermented DM). The pH showed significant differences (P<.0001) between treatments. The rumen digestibility of DM and OM was higher (P=0.0001) in the treatment TPS (45.6 and 45.1% respectively). The proportion (mol/100mol) of volatile fatty acids, no showed significant differences between treatments evaluated (P>0.05). It can be concluded that the incorporation of additives such as enzymes and S. cerevisiae in fibrous forages of Musa spp can benefit the rumen functions, increasing digestion and mitigating the production of greenhouse gases in ruminants.