Efecto antimicrobiano in vitro del aceite esencial de eucalipto (Eucalyptus spp.) sobre cepas certificadas de Escherichia coli Y Staphylococcus aureus

Abstract

The objective of the research project was to evaluate in vitro the antimicrobial effect of Eucalyptus essential oil (Eucalyptus spp.) on certified strains of Escherichia coli ATCC25922 and Staphylococcus aureus subsp. aureus ATCC292113; in which dilutions of eucalyptus oil were evaluated 30%, 60% and 90% with 98% ethanol. The Minimum Inhibitory Concentration was determined using the broth microdilution method, each bacterial inoculum was standardized to 0.5 of the MacFarland scale in the spectrophotometer, obtaining as a result that the 60% and 90% eucalyptus oil tubes did not present turbidity for Escherichia coli, similar characteristic that presented the strain Staphylococcus aureus subsp. aureus in the 60% and 90% dilutions, the Bactericidal Minimum Concentration was also determined by seeding the dilutions mentioned above in the Mueller-Hinton agar in which no growth of colonies was observed at 60% and 90% for the two strains evaluated. The disc diffusion test showed the sensitivity of the evaluated strains to eucalyptus oil, presenting for Escherichia coli a minimum halo of 10.25mm at 30% and a maximum diameter of 10.95mm at 90% while for the Staphylococcus aureus strain subsp. aureus obtained a minimum value of 10.95mm at 30% and a maximum value of 14.45mm at 60% dilution. Eucalyptus essential oil (Eucalyptus spp.) Has antibacterial properties for Gramnegative Escherichia coli and Gram-positive bacteria Staphylococcus aureus sbsp.aureus.