Influencia de la adición de enzimas exógenas y Saccharomyces cervisiae en las dietas de ovinos sobre la fermentación ruminal in vivo E in vitro de forrajes fibrosos

Abstract

The objective of the present investigation was to evaluate the effect of exogenous enzymes and Saccharomyces cerevisiae in four fibrous forages (barley, oats, corn panca, quinoa panca) on ruminal fermentation in vivo and in vitro in sheep. For this, 4 male crossbred (whole) male sheep were used (Bar Diamond branded cannula, Parma, Idaho, USA) of 2 inches of internal diameter and 5 inches of external diameter). The animals were housed in individual cubicles and fed a diet based on barley that met the nutritional requirements, this diet was included exogenous fibrolytic enzymes and Saccharomyces cervisiae under the following doses or treatments: T1: 0% (enzymes or yeasts ), T2: diet with 0.2% enzymes, T3: diet with 0.09% yeast, T4: diet with 0.2% enzyme + 0.09% yeast. The ruminal functions evaluated were in vitro digestibility, kinetics of ruminal degradation in vivo and in vitro gas production. It was observed that the in vitro digestibility of MS and MO showed difference (P <0.05) between the treatments. In barley, greater digestibility index was obtained in treatments T4, and T3 (P = 0.0242 and 0.0204 respectively). In the oats in T2, and T4 (P = 0.0098 and 0.0060). In the corn pan in T1 and T4 (P = 0.0111 and 0.0128) and in the quinoa panca in T2 and T3 (P = 0.0061 and 0.0052). The production of in vitro gas in MS and MO shows statistical differences between rumen liquids. Obtaining as a result that in barley and corn pan the treatments that have less index of accumulated gas production ml / 0.5g MS fermentable (GV) is T1 and T4 (P = <.0001). However, in oats the most effective were T1 and T2 (P = <.0001) and finally in the quinoa pancake T1 (<.0001). In barley the treatments that have less production of methane gas (PM) is T2 and T1(P = <.0001). In oats T2, T3 and T4 (P = 0.0003). In the corn pan T3 and T4 (P = 0.0009) and in the quinoa panca T2 and T3 (<.00460).