Obtención de concentrados proteicos de alpiste (Phalaris canariensis) y evaluación de su capacidad inhibitoria de la peroxidación lipídica en larvas de pez Cebra (Danio rerio)

Abstract

The present research focused on obtaining protein concentrates of canary seed for evaluation of its antioxidant activity. The concentrates were obtained by isoelectric precipitation at pHs of solubility 8.0 and 10.0 and pHs of precipitation 3.0; 5.0 and 6.0; Obtaining the highest yield in the treatment 10.0-6.0. The protein content of bird meal was 17.64 percent; The concentrate was 48 percent; approximately. Polyphenol content values of 78.75 to 182.09 mg GAE per 100 g were determined in the supernatants obtained from the isoelectric precipitation of the proteins. Protein digestibility was determined by in vitro assays and using the SDS-PAGE technique. Proteins were partially digested in gastric digestion at pH 1.2; 2.0 and 3.2. In the duodenal digestion they were fully digested in the hydrolysates from the gastric digests at pHs 1,2 and 2,0. By electrophoresis, the protein profile of the canary seedlings was found, with bands with molecular weights ranging from 11 to 75 kDa, corresponding to globulins and albumins. The chromatographic profile of the protein concentrates and the hydrolysates were obtained by RP-UHPLC; Being able to observe the degree of hydrolysis in the different treatments. The antioxidant activity of the proteins was determined by in vitro and in vivo assays. In the in vitro test, values between 75.5 and 81.31 percent of antioxidant activity were observed. The in vivo test in zebrafish larvae was obtained in a value of 20.5 percent of antioxidant activity.