Carrera de Biotecnología

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    Evaluación de la capacidad fitorremediadora de Chrysopogon zizanioides y Eichhornia crassipes en efluentes secundarios de una curtiembre de la provincia de Tungurahua. Articulado al Proyecto de Investigación aprobado mediante Resolución Nro. UTA-CONIN-2023-0194-R
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Escobar Jumbo, Bruno Manuel; Flores Tapia, Nelly Esther
    Since the 20th century, the increasing global demand for leather has driven the tanning industry to boost its production in Ecuador. However, this industry is one of the most polluting due to the generation of untreated waste, creating an environmental issue on a global scale. This study aims to evaluate the capacity of a phytoremediation process using Eichhornia crassipes (water hyacinth) and C. zizanioides (Vetiver) to treat a contaminated effluent in the city of Ambato, Tungurahua province. To achieve this, three treatments were formulated in artificial wetlands, with a control and three replicates: T1-E. crassipes, T2-C. zizanioides, and T3-Combination (50:50). Over 45 days, physicochemical analyses were conducted on 14 parameters following TULSMA standards, using Hach, Hanna Instruments, and Velp Scientifica reagents. The results show a significant reduction of contaminants in treatment T3 with the following data: BOD5 84 percent, Cr6+ 75 percent, TSS 61 percent, Nitrogen 71 percent, Ammonium 60 percent, Turbidity 99 percent, Color 79 percent, and Phosphorus 60 percent. Additionally, an initial and final characterization encompassing 34 physicochemical parameters was performed. The obtained results demonstrate the effectiveness of the treatment with the combination of both plant species in an artificial wetland. This provides a starting point for studying the interaction of E. crassipes and C. zizanioides in tannery effluents and formulating treatments that help mitigate the environmental impact of the leather and dyeing industry.
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    Evaluación de la eficacia combinada de Eichhornia crassipes y Salvinia natans para descontaminar el agua de descarga de la PTAR Artezón-Píllaro mediante fitorremediación
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Aucapiña Chango, Grace Diana; Flores Tapia, Nelly Esther
    Wastewater from the Wastewater Treatment Plant (WWTP) contains organic compounds, salts, and heavy metals that pose an environmental threat when released without complying with the TULSMA regulations. This affects water quality and aquatic life, necessitating an efficient phytoremediation system for its decontamination. The objective of this work was to evaluate the effectiveness of a phytoremediation system using combinations of E. crassipes and S. natans to decontaminate wastewater. The methodology included an experimental phase with different proportions of E. crassipes and S. natans in 25 liters of water. Physicochemical and microbiological parameters were characterized at 0, 7, 15, 30, and 45 days using oxitop methods, gravimetry, and adapted Hanna protocols based on the Standard Methods for the Examination of Water and Wastewater. The results demonstrated that combinations of E. crassipes and S. natans achieved high contaminant removal rates, including percentages of 97.5 COD, 97.88 BOD, 99.47 ammoniacal nitrogen, 98.82 total phosphorus, 93.99 surfactants, 82.68-93.85 total solids, 64.19 color, 64.76 conductivity, 98.68 turbidity, 99.60 copper, 92.30 aluminum, 79.67 chromium, 99.57 total nitrogen, 80 oils and greases, and 95.24 fecal coliforms. The application of the wastewater treatment system will enable the WWTP waters to comply with regulations. This work demonstrates the feasibility and effectiveness of a natural and sustainable approach to wastewater decontamination, contributing to improved water quality and ecosystem protection.
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    Evaluación de la capacidad fitorremediadora del Eichhornia crassipes y Salvinia natans en aguas residuales de la PTAR Artezón -Píllaro
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Chiriboga Villagomez, Viviana Nataly; Flores Tapia, Nelly Esther
    This study examines water pollution exacerbated by industrial and urban discharges, affecting the quality of water essential for agricultural irrigation and health. Therefore, the phytoremediation capacity of the species S. natans and E. crassipes in the wastewater of the Artezón Píllaro WWTP was evaluated. During the acclimatization process, both plants demonstrated their suitability for phytoremediation, achieving a reduction in COD with S. natans and E. crassipes. Treatments with different masses were applied: for E. crassipes, M1 0-500 g, M2 501-700 g, and M3 (701-800 g); and for S. natans, M1 (0-400 g), M2 (401-800 g), and M3 (801-900 g). Physicochemical and microbiological parameter analyses were performed at intervals of 0, 7, 15, 30, and 45 days, following the protocols of the Hanna manual and the Standard Methods for the Examination of Water and Wastewater. The results showed that both plants reduce the levels of organic pollutants and heavy metals in the wastewater, varying according to the mass used and the type of plant. For E. crassipes, the M1 treatment showed high removal percentages in surfactants, total solids, ammoniacal nitrogen, COD, BOD, turbidity, color, chromium VI, copper, aluminum, total nitrogen, fecal coliforms, and total phosphorus. On the other hand, S. natans with the M3 treatment also showed efficient removal percentages in surfactants, total solids, ammoniacal nitrogen, COD, BOD, turbidity, color, chromium VI, copper, aluminum, total nitrogen, fecal coliforms, and total phosphorus.
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    Evaluación del tratamiento de electrocoagulación seguido del proceso de fitorremediación de Eichhornia crassipes en aguas residuales de curtiembre de la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Ortiz Tibán, Pamela Alejandra; Flores Tapia, Nelly Esther
    Tannery wastewater, with its complex mixture of organic compounds, salts and heavy metals, constitutes a serious environmental threat when released without adequate treatment, negatively affecting water quality and aquatic life. This project focuses on the evaluation of a combined treatment of electrocoagulation and phytoremediation with Eichhornia crassipes in wastewater from a tannery in Tungurahua. The methodology used involved the initial characterization of physicochemical parameters with Standard Methods for the Examination of Water and Wastewater using the HANNA equipment. The treatment was divided into two phases: electrocoagulation as pretreatment and phytoremediation with different masses of E. crassipes (138, 217 and 520) grams. For two months, a detailed monitoring of the decontamination was carried out, evaluating the parameters according to the TULSMA regulations. Preliminary results revealed that electrocoagulation achieved significant reductions of 60 to 80 percent in several contaminants. Phytoremediation, especially with E. crassipes M3, proved to be highly effective in the absorption and accumulation of heavy metals, achieving notable removals of 90 to 100 percent. This combined approach emerges as a promising solution for tannery wastewater treatment, highlighting the importance of technology integration to address the complexity of these industrial effluents. The project not only contributes to environmental mitigation but also to the implementation of sustainable strategies in industrial wastewater management.