Carrera de Biotecnología

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800

Browse

Subject: search.filters.subject.DOXICICLINA

Search Results

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    Eficiencia del almidón de chonta (Bactris gasipaes) como agente encapsulante sobre la actividad antimicrobiana de la doxiciclina
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Aponte Urrutia, Kerly Anais; López Hernández, Orestes Darío
    Doxycycline is an antibiotic of great interest in the health area, since it serves as a treatment for a variety of bacterial infections, but it has a drawback since it tends to degrade very easily at the time of its production, that is why new alternatives have been sought to preserve its bioactive components and protect it from contamination and even increase its bioavailability with the help of microencapsulation which is a technique that uses encapsulating materials such as natural polymers in order to generate greater effectiveness in encapsulation. For this, three suspensions were made with different proportions, using analysis techniques and verification of microencapsulation efficiency which were, performance analysis, statistical analysis, thermograms by means of differential scanning calorimetry (DSC) and antimicrobial evaluation, which was carried out by means of antibiograms of diffusion disks in Müller Hinton agar, to determine the inhibition of the microencapsulate against the E. coli bacteria. Through the different analyses carried out, it was obtained that the best performance and inhibition halos were given in the proportion 20:80, in addition to this, through the thermograms it was possible to observe the melting peaks where it was also represented that the best curve was that of the proportion 20:80. This is thanks to the fact that with a higher polymeric load, the percentage of microencapsulation efficiency increases. This could also be observed with the help of an-ANOVA analysis, which allowed the most significant performance to be seen through statistical graphs.
  • No Thumbnail Available
    Item
    Elaboración de liposomas por combinaciones de fosfolípidos para la encapsulación del antibiótico doxiciclina como sistema de administración de fármacos
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Cruz Vasquez, Josué David; Núñez Villacís, Lorena de los Ángeles
    The development of nanodrug delivery systems is important for the better treatment of diseases. The objective of this study was to prepare liposomes with lecithin (CL) and cholesterol (CHO) for the encapsulation of the antibiotic doxycycline. This antibiotic is important in the treatment of bacterial infections and is administered orally. The preparation of the liposomes was carried out using the hydrated film method, evaluating different proportions of CL:CHO. Liposomes were characterized by infrared analysis (FT-IR), thermal analysis (DSC), and transmission electron microscopy (TEM). Additionally, the stability of the liposomes in the digestive tract was evaluated through an in vitro simulation and also the best storage temperature was tested. It was determined that the ideal proportion of CL:CHO to obtain the liposomes was 70:30, this same proportion was the one that presented the best doxycycline encapsulation efficiency with 56 percent. The formation of spherical nanovesicles and the presence of chemical groups of each component were verified in the liposomes loaded with doxycycline. The liposomes maintained an average stability of percent, close to liposomes without digestion, and the best storage temperature was 4 degrees Celsius after two weeks. In conclusion, it has been shown that with CL and CHO are good elements for liposomes fabrication and drug encapsulation, such as doxycycline. These liposomes achieve an adequate encapsulation percentage and stability, which is why they have the potential to be applied as nanosystem for oral drug administration.
  • No Thumbnail Available
    Item
    Elaboración de liposomas por combinaciones de fosfolípidos para la encapsulación del antibiótico doxiciclina como sistema de administración de fármacos
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Chávez Salazar, Hérman Alexis; Núñez Villacís, Lorena de los Ángeles
    The development of nanodrug delivery systems is important for the better treatment of diseases. The objective of this study was to prepare liposomes with lecithin (CL) and cholesterol (CHO) for the encapsulation of the antibiotic doxycycline. This antibiotic is important in the treatment of bacterial infections and is administered orally. The preparation of the liposomes was carried out using the hydrated film method, evaluating different proportions of CL:CHO. Liposomes were characterized by infrared analysis (FT-IR), thermal analysis (DSC), and transmission electron microscopy (TEM). Additionally, the stability of the liposomes in the digestive tract was evaluated through an in vitro simulation and also the best storage temperature was tested. It was determined that the ideal proportion of CL:CHO to obtain the liposomes was 70:30, this same proportion was the one that presented the best doxycycline encapsulation efficiency with 56 percent. The formation of spherical nanovesicles and the presence of chemical groups of each component were verified in the liposomes loaded with doxycycline. The liposomes maintained an average stability of percent, close to liposomes without digestion, and the best storage temperature was 4 degrees Celsius after two weeks. In conclusion, it has been shown that with CL and CHO are good elements for liposomes fabrication and drug encapsulation, such as doxycycline. These liposomes achieve an adequate encapsulation percentage and stability, which is why they have the potential to be applied as nanosystem for oral drug administration.