Carrera de Biotecnología

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800

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Subject: search.filters.subject.ACTIVIDADES BIOLÓGICAS

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    Evaluación de la actividad biológica de los compuestos bioactivos fenólicos del extracto etanólico de Anís (Pimpinella anisum)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Urbina Monroy, Diana Carolina; Pérez Aldás, Lander Vinicio
    This study evaluated the biological activity of phenolic compounds present in the ethanolic extract of Pimpinella anisum (green anise). The Soxhlet extraction method was used using 96 percent ethanol, obtaining a yield of 8 percent. The results revealed a total phenol concentration of 76.2 mg GAE per ml. The antioxidant capacity was evaluated by the DPPH and ABTS methods, showing a maximum inhibition of 66.47 percent and 76.32 percent, respectively, indicating that the extracts have the potential to neutralize free radicals. In addition, the anti-inflammatory capacity was determined by denaturation of egg albumin and bovine albumin, reaching maximum inhibitions of 68 percent and 78.48 percent respectively. These results suggest that the phenolic compounds of P. anisum possess significant antioxidant and anti-inflammatory activity, comparable with other plant extracts. Statistical analysis using ANOVA and Tukey's test confirmed significant differences between the tested concentrations. The findings highlight the potential of P. anisum as a natural source of bioactive compounds with applications in various industries. Further detailed analysis is recommended to identify the specific compounds responsible for these biological activities and validate these results in vivo. The importance of implementing sustainable practices in the cultivation and harvesting of the plant to preserve its quality is also highlighted.
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    Identificación de la capacidad biológica del extracto etanólico de hojas de menta (Mentha arvensis)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Paredes Manzano, Johnny Andrés; Pérez Aldás, Lander Vinicio
    The use of natural substances derived from medicinal plants has been a widespread practice throughout history, offering valuable therapeutic alternatives. In Ecuador, a megadiverse country with a rich flora, there is a growing interest in exploring these plants as sources of bioactive compounds for medical and pharmaceutical applications. This work focuses on the evaluation of the biological properties of the ethanolic extract of mint leaves (Mentha arvensis) in Ecuador, in order to study its potential as a source of natural bioactive compounds and explore its therapeutic application. The ethanolic extract of mint leaves was obtained by the Soxhlet method whose yield was 14.55 percent. The determination of total phenols was performed by the Folin-Ciocalteu assay and the evaluation of its antioxidant activity by the DPPH and ABTS assays. In addition, its anti-inflammatory capacity was evaluated by protein denaturation assays (egg albumin and bovine albumin). The results obtained showed that the extract of M. arvensis has a high content of total phenols of 57.59 mg GAE per gran of extract, and also exhibits a significant antioxidant activity of 86.36 and 82.78 percent for the DPPH and ABTS assays respectively, being a potential as a source of natural antioxidant agents. Likewise, a remarkable anti-inflammatory activity of 67.54 and 71.31 percent was observed for the egg albumin and bovine albumin denaturation assays respectively, indicating to be effective in the treatment of inflammatory conditions.
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    Determinación de la actividad biológica de los compuestos bioactivos del extracto etanólico de Hierba Luisa (Cymbopogon citratus)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Molina Alomaliza, Joselyn Stefany; Pérez Aldás, Lander Vinicio
    Cymbopogon citratus, commonly known as Lemongrass, is a herbaceous plant of the Poaceae family native to tropical and subtropical regions. Widely valued in traditional medicine, multiple therapeutic properties are attributed to it, including antispasmodic, antimicrobial, antitussive, antipyretic, anti-inflammatory and sedative effects. Although the scientific evidence that supports these benefits is still limited and it is unknown if they would have the same composition in Ecuadorian territory. Furthermore, Lemongrass is not considered a substitute for conventional medical treatments, more research is required to confirm its medicinal properties. In this study, the biological activity of the bioactive compounds from the ethanolic extract of Lemongrass was evaluated. The ethanolic extract of C. citratus was extracted using the Soxhlet method. Subsequently, a stock solution was prepared by dissolving 1 g of extract in 100 mL of distilled water, obtaining dilutions of 1:10, 1:100 and 1:1000 used in all tests. Total phenolic properties were measured with the Folin-Ciocalteu method, reaching 76.24 mg GAE per g of extract at the highest concentration. The antioxidant capacity was evaluated using the DPPH and ABTS methods, obtaining the maximum values of 459.81 and 690.35 mgTE per g of extract, respectively. To evaluate the anti-inflammatory capacity, two methods were used: egg albumin denaturation and bovine serum albumin denaturation. Both confirmed the anti-inflammatory activity of the extract, with the egg albumin method being superior in sensitivity and reproducibility. This study highlights medicinal properties of C. citratus, reaffirming its potential in pharmacological applications.