Carrera de Biotecnología

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    Aplicación de la tecnología de metabarcoding 16S rRNA para el análisis de la rizosfera de mora (Rubus glaucus) en cultivos de la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Vallejo Guerrero, Lizbeth Andrea; Calero Cáceres, William Ricardo
    This study focuses on characterizing the diversity and abundance of the microbiota associated with the blackberry rhizosphere, as well as predicting the metabolism of the taxa found. Utilizing next-generation sequencing (NGS) technology and analysis of the hypervariable regions V3-V4 of the 16S rRNA gene, a wide range of bacterial taxa predominantly Proteobacteria, Firmicutes, and Actinobacteria were identified. Alpha and beta diversity analyses revealed high microbial diversity in most of the sampled locations, with significant variations between sampling points, indicating the influence of local environmental factors. Additionally, functional predictions of metabolic profiles were performed using advanced bioinformatics tools such as PICRUSt2 and EzBioCloud, highlighting the contribution of these microbial communities to the biogeochemical cycles of nitrogen, carbon, and sulfur. The results underscore the importance of rhizospheric microbiota in soil health and the growth of R. glaucus and suggest its potential for use in biocontrol and biofertilization strategies. This study not only provides a deeper understanding of microbial ecology in blackberry crops but also offers a foundation for future research and practical applications in sustainable agricultural management in the Tungurahua region.
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    Aplicación de la tecnología de metabarcoding 16S rRNA para el análisis de la rizosfera de mora (Rubus glaucus) en cultivos de la provincia de Tungurahua
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Corrales Iza, Lenin Apurimak; Calero Cáceres, William Ricardo
    This study focuses on characterizing the diversity and abundance of the microbiota associated with the blackberry rhizosphere, as well as predicting the metabolism of the taxa found. Utilizing next-generation sequencing (NGS) technology and analysis of the hypervariable regions V3-V4 of the 16S rRNA gene, a wide range of bacterial taxa predominantly Proteobacteria, Firmicutes, and Actinobacteria were identified. Alpha and beta diversity analyses revealed high microbial diversity in most of the sampled locations, with significant variations between sampling points, indicating the influence of local environmental factors. Additionally, functional predictions of metabolic profiles were performed using advanced bioinformatics tools such as PICRUSt2 and EzBioCloud, highlighting the contribution of these microbial communities to the biogeochemical cycles of nitrogen, carbon, and sulfur. The results underscore the importance of rhizospheric microbiota in soil health and the growth of R. glaucus and suggest its potential for use in biocontrol and biofertilization strategies. This study not only provides a deeper understanding of microbial ecology in blackberry crops but also offers a foundation for future research and practical applications in sustainable agricultural management in the Tungurahua region.
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    Evaluación de medios de cultivo y fitohormonas para la inducción de callogénesis a partir de dos explantes de arándano (Vaccinium corymbosum L.) cv. Biloxi
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Peralta Lluglla, Oscar David; De la Torre Olvera, Helena Maritza
    Blueberry consumption has increased its demand at international and national level, healthy plants are necessary efficient, therefore, this research seeks to evaluate culture media and phytohormones for the induction of callogenesis from two blueberry explants (Vaccinium corymbosum L) cv. Biloxi. Blueberry leaves and flowers that had undergone a disinfection process were introduced. In addition, leaves from plants established under in vitro conditions were evaluated. Woody plant medium was used as a base to formulate a total of thirteen treatments to which enrichers and different concentrations of phytohormones were added. The percentage of callus induction and the percentage of embryogenic calli, their morphological characteristics and their viability were evaluated. The results showed that the leaves taken ex vivo were not viable for callus development. No significant differences were found in the doses of 2,4D and KIN used in flowers. In leaves, the dose of 2 milligrams per litre of 2,4D gave callus with slightly better characteristics and the concentration of 3 milligrams per litre of KIN also showed a slight favourable difference. In conclusion, the composition of the medium, the hormone interaction, the type of explant and their interactions have a significant effect on the rate of callus induction. Furthermore, the best explant for efficient callus development is the flower, which provides favourable characteristics for a subsequent process of complete differentiation of the blueberry plant.
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    Evaluación de medios de cultivo y fitohormonas para la inducción de callogénesis a partir de dos explantes de arándano (Vaccinium corymbosum L.) cv. Biloxi
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Changoluisa Arguero, Dayanna Mishel; De la Torre Olvera, Helena Maritza
    Blueberry consumption has increased its demand at international and national level, healthy plants are necessary efficient, therefore, this research seeks to evaluate culture media and phytohormones for the induction of callogenesis from two blueberry explants (Vaccinium corymbosum L) cv. Biloxi. Blueberry leaves and flowers that had undergone a disinfection process were introduced. In addition, leaves from plants established under in vitro conditions were evaluated. Woody plant medium was used as a base to formulate a total of thirteen treatments to which enrichers and different concentrations of phytohormones were added. The percentage of callus induction and the percentage of embryogenic calli, their morphological characteristics and their viability were evaluated. The results showed that the leaves taken ex vivo were not viable for callus development. No significant differences were found in the doses of 2,4D and KIN used in flowers. In leaves, the dose of 2 milligrams per litre of 2,4D gave callus with slightly better characteristics and the concentration of 3 milligrams per litre of KIN also showed a slight favourable difference. In conclusion, the composition of the medium, the hormone interaction, the type of explant and their interactions have a significant effect on the rate of callus induction. Furthermore, the best explant for efficient callus development is the flower, which provides favourable characteristics for a subsequent process of complete differentiation of the blueberry plant.