Carrera de Biotecnología

Permanent URI for this collectionhttp://repositorio.uta.edu.ec/handle/123456789/34800

Browse

Filters

20246

Settings

Has files: YesSubject: search.filters.subject.EXTRACTOS ETANÓLICOS

Search Results

Now showing 1 - 6 of 6
  • No Thumbnail Available
    Item
    Análisis de la actividad antiinflamatoria y agregación plaquetaria compuestos bioactivos del extracto etanólico de Cedrón (Aloysia citrodora paláu)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Valencia Armas, Joselin Gabriela; Pérez Aldás, Lander Vinicio
    Currently, access to large medical treatments is a global challenge, which drives a renewed interest in traditional medicine and medicinal plants, however, the lack of scientific studies limits their acceptance in public health. This research analyzes the bioactive compounds of the ethanolic extract of cedron (Aloysia citrodora Paláu) for its anti-inflammatory and antiplatelet aggregation activity. The cedron plant, also known as verbena, confers a range of therapeutic uses including the reduction of inflammation and protection against oxidative stress. Wherein, gallic acid reduces inflammation, while quercetin exhibits anticoagulant properties. The extraction yield was 17.01 percent, and the content of total phenols and flavonoids was found to decrease as the extract was diluted, with maximum values of 163.45 milligram (mg) over gram (g) and 99.12 mg over g, respectively, at the highest concentration. In terms of anti-inflammatory activity, the cedron extract showed an inhibition of albumin denaturation of 61.85 percent, lower than diclofenac (95.1 percent), but comparable as it is diluted. In addition, the extract inhibited platelet aggregation similarly to the control drug at all concentrations tested. The flavonoids and phenols present in the ethanolic extract of cedron exhibit significant anti-inflammatory and antiplatelet aggregation activity, standing out as a natural alternative. These findings contribute to the development of new organic products and to the growth of the Flor Andina company.
  • No Thumbnail Available
    Item
    Identificación de la capacidad biológica del extracto etanólico de hojas de menta (Mentha arvensis)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Paredes Manzano, Johnny Andrés; Pérez Aldás, Lander Vinicio
    The use of natural substances derived from medicinal plants has been a widespread practice throughout history, offering valuable therapeutic alternatives. In Ecuador, a megadiverse country with a rich flora, there is a growing interest in exploring these plants as sources of bioactive compounds for medical and pharmaceutical applications. This work focuses on the evaluation of the biological properties of the ethanolic extract of mint leaves (Mentha arvensis) in Ecuador, in order to study its potential as a source of natural bioactive compounds and explore its therapeutic application. The ethanolic extract of mint leaves was obtained by the Soxhlet method whose yield was 14.55 percent. The determination of total phenols was performed by the Folin-Ciocalteu assay and the evaluation of its antioxidant activity by the DPPH and ABTS assays. In addition, its anti-inflammatory capacity was evaluated by protein denaturation assays (egg albumin and bovine albumin). The results obtained showed that the extract of M. arvensis has a high content of total phenols of 57.59 mg GAE per gran of extract, and also exhibits a significant antioxidant activity of 86.36 and 82.78 percent for the DPPH and ABTS assays respectively, being a potential as a source of natural antioxidant agents. Likewise, a remarkable anti-inflammatory activity of 67.54 and 71.31 percent was observed for the egg albumin and bovine albumin denaturation assays respectively, indicating to be effective in the treatment of inflammatory conditions.
  • No Thumbnail Available
    Item
    Determinación de la actividad biológica de los compuestos bioactivos del extracto etanólico de Hierba Luisa (Cymbopogon citratus)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-08) Molina Alomaliza, Joselyn Stefany; Pérez Aldás, Lander Vinicio
    Cymbopogon citratus, commonly known as Lemongrass, is a herbaceous plant of the Poaceae family native to tropical and subtropical regions. Widely valued in traditional medicine, multiple therapeutic properties are attributed to it, including antispasmodic, antimicrobial, antitussive, antipyretic, anti-inflammatory and sedative effects. Although the scientific evidence that supports these benefits is still limited and it is unknown if they would have the same composition in Ecuadorian territory. Furthermore, Lemongrass is not considered a substitute for conventional medical treatments, more research is required to confirm its medicinal properties. In this study, the biological activity of the bioactive compounds from the ethanolic extract of Lemongrass was evaluated. The ethanolic extract of C. citratus was extracted using the Soxhlet method. Subsequently, a stock solution was prepared by dissolving 1 g of extract in 100 mL of distilled water, obtaining dilutions of 1:10, 1:100 and 1:1000 used in all tests. Total phenolic properties were measured with the Folin-Ciocalteu method, reaching 76.24 mg GAE per g of extract at the highest concentration. The antioxidant capacity was evaluated using the DPPH and ABTS methods, obtaining the maximum values of 459.81 and 690.35 mgTE per g of extract, respectively. To evaluate the anti-inflammatory capacity, two methods were used: egg albumin denaturation and bovine serum albumin denaturation. Both confirmed the anti-inflammatory activity of the extract, with the egg albumin method being superior in sensitivity and reproducibility. This study highlights medicinal properties of C. citratus, reaffirming its potential in pharmacological applications.
  • No Thumbnail Available
    Item
    Evaluación de la vida útil de un extracto obtenido de la cáscara de piña (Ananas comosus) con propiedades antioxidantes y antiinflamatorias
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Nuñez Ambato, Erika Fernanda; Fernández Rivero, Danae
    Considering the growing interest in the utilization of agricultural waste, pineapple peel stands out as a rich source of bioactive compounds. The need to replace synthetic antioxidants and anti-inflammatories, associated with health risks, emphasizes the importance of exploring natural sources. Additionally, the assessment of shelf life is crucial to ensure the stability and efficacy of the extract over time. The extraction process began using ethanol and distilled water as solvents in different proportions. The concentration of polyphenols was determined using the Folin-Ciocalteu method, demonstrating that the ethanol treatment was the most efficient. Total flavonoid evaluation was performed on the extract with the highest polyphenol concentration using the colorimetric method of aluminum chloride. Furthermore, the analysis of antioxidant activity was conducted through the DPPH assay, resulting in an inhibition percentage of 52.27 percent. The analysis of anti-inflammatory activity was also carried out using the egg albumin denaturation assay, revealing significant percentages of denaturation inhibition, reaching 73.15 percent. Stability was analyzed over a month and showed a slight increase in pH and variation in humidity, confirming the robustness of the extract. This study provides valuable information on the shelf life of pineapple peel extract, highlighting its potential as a natural source of antioxidants and anti-inflammatories. This contributes to the expansion of knowledge on the utilization of agricultural by-products and the search for natural solutions to health challenges.
  • No Thumbnail Available
    Item
    Evaluación de la actividad antioxidante e hipoglucemiante in vitro de la combinación de extractos etanólicos de Marco (Ambrosia arborescens), Quishuar (Buddleja incana) y Cedrón (Aloysia citrodora)
    (2024-02) Jaque Pavón, Alison Paola; Pérez Salinas, Ruth Narcisa
    Diabetes Mellitus (DM) is a chronic disease characterized by the high amount of glucose present in the blood, causing hyperglycemia in the patient. One of the factors involved in the progressive development and complications of the disease is the cellular damage produced by the excess of free radicals present in the body. Plants present a great variety of biological properties, conferred by the compounds they possess, such as antioxidant and hypoglycemic activity, given mainly by phenols and flavonoids present in the plant matrix. Therefore, these molecules are studied as alternatives in the treatment of hyperglycemia. This study evaluates the antioxidant and hypoglycemic activity of four combinations of ethanolic extracts of Marco, Quishuar and Cedron. Phenols and flavonoids were quantified using the Folin-Ciocalteu method and the aluminum chloride method. Then, antioxidant and hypoglycemic activity was evaluated by the DPPH method and enzymatic assays with α-glucosidase and α-amylase enzymes. The combination with the highest biological activity was microencapsulated with maltodextrin and a combination of maltodextrin and gum arabic. The Marco-Quishuar (MQ) and Quishuar-Cedron (QC) combinations presented a higher content of the mentioned metabolites. The MQ combination presented a greater inhibition of the DPPH radical and of the α-glucosidase and α-amylase enzymes; therefore, this combination was microencapsulated. By means of infrared spectroscopy (FTIR), the efficiency of the microencapsulation was analyzed. Finally, the combination without microencapsulation exhibited better biological activity.
  • No Thumbnail Available
    Item
    Determinación del efecto ansiolítico in vivo del extracto etanólico de ortiga macho (Urtica leptophylla Kunth) en ratones (Mus musculus)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2024-02) Doicela Pingos, Lizbeth Estefania; Fernández Rivero, Danae
    The research on the anxiolytic evaluation of a native plant from Ecuador, such as the analysis of the anxiolytic potential of the ethanolic extract of Urtica leptophylla Kunth, is crucial for mental health and biodiversity conservation. In a world where anxiety disorders affect an increasing number of people, finding natural and effective alternatives is vital. Additionally, this research merges traditional knowledge with modern science for the benefit of societies and the environment, highlighting the richness of Ecuadorian flora and its potential for sustainable development. The methodology employed included phytochemical tests that revealed the significant presence of phenols and flavonoids, supporting the antioxidant activity of the extract. The use of the Elevated Plus Maze test in mice (Mus musculus) to assess the anxiolytic potential of the extract from Urtica leptophylla Kunth. The exploration of the mice hippocampus response through biochemical tests (DPPH and Bradford) after the behavioral phase provided valuable insights into the extract's ability to counteract oxidative stress in the brain induced by immobilization stress. The results suggest a positive anxiolytic effect comparable to clonazepam, in addition to neuroprotective properties due to its antioxidant capacity. These findings pave the way for investigating and applying natural compounds in the treatment of mental illnesses and brain protection against oxidative stress. In summary, the study provides a comprehensive understanding of the extract's properties and its potential for treating anxiety disorders and protecting the brain.