Browsing by Author "Villacis Gamboa, David Alexander"

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    Evaluación de la sensibilidad antibiótica y presencia de genes de resistencia en Enterococcus gallinarum aislado en carne de pollo en Ambato
    (2024-08) Villacis Gamboa, David Alexander; Cruz Quintana, Sandra Margarita
    Enterococcus gallinarum is a Gram-positive bacterium that grows in short chains and can be found in the gastrointestinal tract of animals and humans at temperatures ranging from 10 - 37ºC. The aim of the study was to evaluate the antibiotic sensitivity and presence of resistance genes in Enterococcus gallinarum isolated from chicken meat in Ambato. The Kirby Bauer method with antibiotic discs was used to evaluate the sensitivity and resistance to vancomycin, erythromycin, ciprofloxacin, amoxicillin + clavulanic acid and trimethopin + sulfamethasone. The antibiotic chosen to detect resistance genes was vancomycin by DNA extraction, PCR and electrophoresis. PCR primers VanA F and VanA R were used to amplify a band of 732 base pairs. Resistance to vancomycin, erythromycin, ciprofloxacin and sensitivity to amoxicillin + clavulanic acid and trimethopin + sulfamethasone was shown. No vancomycin resistance genes were detected and the main molecular mechanisms of resistance of Enterococcus gallinarum to vancomycin were investigated as efflux pumps, resistance genes and target site modification. It was concluded that Enterococcus gallinarum from chicken meat sold in the canton of Ambato is sensitive to clavulanic acid and trimethopin + sulfamethasone and resistant to vancomycin, erythromycin and ciprofloxacin. To vancomycin it does not present resistance genes, its main resistance mechanisms are resistance genes, efflux pumps, modification of the target point.