Browsing by Author "Urbina Monroy, Diana Carolina"

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    Evaluación de la actividad biológica de los compuestos bioactivos fenólicos del extracto etanólico de Anís (Pimpinella anisum)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Urbina Monroy, Diana Carolina; Pérez Aldás, Lander Vinicio
    This study evaluated the biological activity of phenolic compounds present in the ethanolic extract of Pimpinella anisum (green anise). The Soxhlet extraction method was used using 96 percent ethanol, obtaining a yield of 8 percent. The results revealed a total phenol concentration of 76.2 mg GAE per ml. The antioxidant capacity was evaluated by the DPPH and ABTS methods, showing a maximum inhibition of 66.47 percent and 76.32 percent, respectively, indicating that the extracts have the potential to neutralize free radicals. In addition, the anti-inflammatory capacity was determined by denaturation of egg albumin and bovine albumin, reaching maximum inhibitions of 68 percent and 78.48 percent respectively. These results suggest that the phenolic compounds of P. anisum possess significant antioxidant and anti-inflammatory activity, comparable with other plant extracts. Statistical analysis using ANOVA and Tukey's test confirmed significant differences between the tested concentrations. The findings highlight the potential of P. anisum as a natural source of bioactive compounds with applications in various industries. Further detailed analysis is recommended to identify the specific compounds responsible for these biological activities and validate these results in vivo. The importance of implementing sustainable practices in the cultivation and harvesting of the plant to preserve its quality is also highlighted.