Browsing by Author "Sánchez Guevara, Jazmine Estefania"

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Desarrollo de un método para la extracción de cannabidiol (CBD) a partir de flores de Cannabis sativa L. en el Laboratorio Neofarmaco del Ecuador CIA. LTDA.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2023-09) Sánchez Guevara, Jazmine Estefania; Tubón Usca, Irvin Ricardo
    The development of new pharmaceutical products in laboratories suggests the rigorous quality control of these, especially of plant matrices such as Cannabis sativa L. The present project developed a method for the extraction of cannabidiol (CBD) from two types of flowers (untrimmed and trimmed). For which the different types of flowers were evaluated based on their organoleptic and physicochemical characteristics, yield and amount of CBD considering parameters of the United States Pharmacopoeia (USP). Both types of flowers presented adequate morphological (color, aroma) and physicochemical (humidity, variety of trichomes) characteristics. However, the untrimmed flower presented a better extraction yield of 38.761 percent under decarboxylation and freezing conditions using 25 grams of plant material and 165 milliliters of ethanol for 24 days. In addition, the HPLC validation showed a correct linearity, precision (repeatability), limit of detection and quantification and suitability of the CBD standard. ANOVA also showed similar amount of CBD in the ethanol extract with values of 2.101 and 2.056 grams when the untrimmed flower was used under temperatures of -10 and 25 degrees Celsius respectively. The results support the suitability of the method using both types of flowers. However, to obtain higher yield and quantity of CBD it is ideal to use the untrimmed flower considering lower extraction temperatures, as well as longer decarboxylation times and temperatures. Finally, the method can be included in the quality control analysis in the Neofarmaco Laboratory.