Browsing by Author "Ortiz Pérez, Tatiana Anabel"

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Aprovechamiento de las propiedades de Oxalis lotoides (Kunth) y sus potenciales usos industriales
(Universidad Técnica de Ambato. Facultad de Ingeniería en Sistemas, Electrónica e Industrial. Maestría en Química, 2024) Ortiz Pérez, Tatiana Anabel; Pérez Aldás, Lander Vinicio
The use of medicinal plants in Ecuador has shown constant growth, this growth is due to the therapeutic value that plants have according to their composition and active ingredients. These principles are involved in highly relevant biological processes and can be used in various industrial processes to obtain functional products. The aim of the research was to study the antioxidant and bromatological properties of the leaves and stems of Oxalis lotoides (Kunth) and their use in the formulation of an infusion. The plant material was dried at 70° C, ground to obtain a fine powder and the active ingredients were extracted using the Soxhlet method. The extract obtained was concentrated and stored in amber bottles. The antioxidant activity was determined by the DPPH method, and the bromatological characteristics were also determined by physical-chemical methods. The results for the ethanolic extract being the OLH treatment (Oxalis Lotoides Leaves) was 24.515 % while for the ethanolic extract of stems being the OLT treatment (Oxalis Lotoides Stems) was 50.193 %. The bromatological analysis showed the following values: humidity OLH = 8.190 % and OLT = 6.181 %; crude protein OLH = 18.2 % and OLT = 4.71 %; total fats OLH = 8.48 % and OLT = 4.44 % ashes OLH = 5.143 % and OLT = 5.766 %; titratable acidity citric acid OLH = 1.658 % and OLT = 0.662 %; pH OLH = 1.74 and OLT = 2.72 and soluble solids OLH = 4.157 % and OLT = 2.90 %. With the ethanolic extracts of leaves and stems, the microencapsulation process was carried out by spray drying with an inlet and outlet temperature of 150 ° C and 80 ° C respectively, with these microencapsulates the aromatic infusions were made which were evaluated by a panel of students using a hedonic scale, it was obtained that the infusion with the best characteristics of flavor, smell, color, appearance and acceptability was the OLH treatment.
Evaluación de la actividad antioxidante, antiinflamatoria y citotóxica in vitro de diferentes extractos de Chuquiragua (Chuquiragua jussieui) obtenidos mediante secado por aspersión
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-03) Ortiz Pérez, Tatiana Anabel; Pérez Betancourt, Yunys
The unsuitable used the medicinal plants in Ecuador the search new alternatives for reducing these problems such as get active compounds through technical process and the use endemic plants of Ecuador. The aim of present investigation was to evaluate active compounds showed in the vegetal extracts of Chuquiragua of different in vitro biological activities. The extract got the leaves and flowers through a maceration process with different solvents such as: distillated water and ethanol. Through the spray drying method got a compact powder of different extracts. The biological activities evaluate through in vitro method, the anti-inflammatory activity there were used erythrocytes healthy patients review the stability membrane of giving extract avoid to start inflammatory process. The cytotoxic activity was quantified through a colorimetric method of de bromide de 3-(4,5-dimetiltiazol-2-ilo)-2,5-difeniltetrazol (MTT) with cellular lineal of breast cancer MFC-7, and serial dilutions. In anti-inflammatory, antioxidant and cytotoxic activities, the aqueous ethanol leaves dried of sun was the best treatment with these results 92,602% of the protection, 147409,481 μg Gallic acid/g sample and 98,913% of inhibition cellular respectively. In conclusion, flowers and leaves of Chuquiragua showed biological activities that through pharmacological processes might to active compounds. These maybe use in medical ambit.