Browsing by Author "Llambo Charco, Darwin Marcelo"

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    Análisis de la capacidad antimicrobiana de un péptido obtenido en un sistema de expresión Escherichia coli J53 frente a cepas de Staphylococcus spp.
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Biotecnología, 2025-02) Llambo Charco, Darwin Marcelo; Garcés Moncayo, María Daniela
    Pathogenic microorganisms of the Staphylococcus spp. genius have represented one of the main causes of infections in nosocomial environments, immunosuppressed population, and patients with medical devices. The S. aureus strain has been particularly worrying due to its increasing resistance to antibiotics such as methicillin (MRSA) and certain beta-lactams such as cephalosporin and penicillin due to the presence of the mecA gene. Obtaining proteins from expression systems is a viable alternative for the development of new therapeutic avenues for infections caused by the Staphylococcus spp. genus. This research project was developed with the aim of analyzing the antimicrobial capacity of the peptide Alpha-1-Purothionine obtained in an E. coli J53 expression system against strains of S. aureus and coagulase-negative S. (CoNS). The process began with the cellular chemically competent of the host, induction with IPTG and the analysis of the expression by means of SDS PAGE, where a certain level of basal expression is evident. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated. Finally, it was determined that the peptide presented antimicrobial properties, inhibiting the growth and development of the S. aureus and CoNS strains through colorimetric verification using the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), showing values of 8,5 micrograms per millimeters for S. aureus and 17,0 micrograms per millimeters for CoNS.