Browsing by Author "Iler Iler, Diana Carolina"

Now showing 1 - 1 of 1

Evaluación de la actividad nematicida in vitro de aceites esenciales frente a Meloidogyne
(Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos. Carrera de Ingeniería Bioquímica, 2017-10) Iler Iler, Diana Carolina; Arancibia Soria, Mirari Yosune
Plant-parasitic nematodes from the genus Meloidogyne attack a great number of species of plants and their control is based on the application of chemical products which causes problems for the people and the environment. Therefore organics alternatives are being sought. In this sense the essential oils whit proven biocide activity against microorganisms such as fungi, bacteria and even insects, represent a viable alternative to the use of chemical nematicidas. In this research was evaluated the nematicidal activity of 4 types of essential oil (oregano, thyme, eucalyptus y rosemary) at three different concentrations (0.25 percent, 0.50 percent, 0.75 percent v/v) against second stage juvenile (J2) of Meloidogyne spp extracted from tomato (Lycopersicon esculentum) roots, during 48 hours at different time periods. Although, all essential oils showed higher nematicidal activity at 0.75 percent concentration, essential oils from oregano and thyme were significantly better than those of eucalyptus and rosemary, achieving a mortality percentage of 100 percent after 8 hours of exposure for the oregano essential oil and 100 percent mortality at 24 hours for thyme. In addition, damage was evaluated in J2 nematodes exposed to essential oils by means of light microscopy and scanning electron microscopy. The structure of the nematodes showed dark vacuoles and internal organ damage. The excellent results obtained with two of the essential oils studied offer encouraging possibilities to become an alternative to the use of chemical nematicides in the agricultural industry