Browsing by Author "Escobar Reyes, Mercy Jeaneth"

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    Caracterización de levaduras aisladas a partir de frutos de durazno (Prunus persica), fresa (Fragaria vesca) y manzana (Malus domestica)
    (Universidad Técnica de Ambato. Facultad de Ciencia e Ingeniería en Alimentos y Biotecnología. Carrera de Ingeniería Bioquímica, 2019-07) Escobar Reyes, Mercy Jeaneth; Rodríguez Meza, Carlos Alberto
    This paper shows the importance of the phenotypic and genotypic characterization for the identification of yeasts that were isolated from seasonal fruits peach, strawberry and apple from three different locations Montalvo-Palagua, Huachi Grande-San Alfonso, Alobamba – the triumph. The time, yeasts have generated a biotechnological interest due to their application in the industry, rapid growth and biomass production. The fermentation of the fruits made before the isolation allowed to obtain differences in the number of CFU for every ml according to the fruit and place of collection. A total of ninety four isolated and conserved strains were obtained. For the identification of these was taken different criteria as morphological where analyzed their characteristics macroscopic and microscopic, physiological criteria of growth in different ranges of pH, salinity, temperature and assimilation of Amino acids as a source of carbon and nitrogen. The characterization genotypic was performed by analyzing the digestion of the region ITS by means of RFLP, the results of numerical taxonomy obtained were analyzed in the statistical software NTSys which allowed to obtain a taxonomic determination with which it was possible to group and form sixteen species groups resulting from the analysis of numerical taxonomy of phenotypic data and fourteen species groups resulting from the numerical taxonomy of genotypic data. The final analysis of taxonomy Polifásica made for fifty-four strains of yeast where they grouped their characteristics resulting from the analysis phenotypic and Genotypic obtaining a final dendogram with a cut to 80 percent of similarity that allowed Obtain 18 species groups where it is observed its wide distribution for the fruits and places of where they were isolated the yeasts.