Evaluación in vitro del efecto antimicrobiano de la miel de eucalipto sobre Mammaliicoccus sciuri

Abstract

The objective of this research work was to determine the antimicrobial effect of eucalyptus honey at different concentrations on cultures of Mammalicococcus sciuri. The first stage of the study consisted of the physicochemical characterization of eucalyptus honey, in order to establish those factors that determine its antimicrobial properties, the following results were obtained: Electrical conductivity (0.64 mS /cm), Humidity (14.80 %), Light amber color (50.40 mmPFund), Ash (0.46 %), pH (5. 43), HMF (11.83 mg HMF / kg), Diastase index (22.71 °S), Phenols (80.15 GAE / 100 g) and Flavonoids (1.49 mg QE / 100 g). In the second stage, the antimicrobial effect of eucalyptus honey was tested using the diffusion method in modified agar in wells, and different dilutions of eucalyptus honey were compared on M. sciuri in order to find the minimum active dilution (MAD) at which bacterial growth is inhibited, determining a MAD value of 15%. The third stage of this study consisted of defining the influence of eucalyptus honey on colony forming units (CFU) in the brain heart agar culture medium and comparing it with an artificially prepared sugar solution that pretends to imitate the chemical composition of honey, observing values such as: for eucalyptus honey (3.85 x 10 6 CFU/ml) and for the sugar solution (6.42 x 10 6 CFU/ml), evidencing the antimicrobial effect of eucalyptus honey on M. sciuri. Key words: Mamaliicocus sciuri, antimicrobial effect, honey, eucalyptus.