Evaluación de la actividad antioxidante de extractos de Aloysia citrodora (Cedrón) y Prunus serotina (Capulí) en Andamios biológicos

Abstract

Ischemic heart disease (IHD) is a disease caused by obstruction of blood flow, due to factors such as oxidative stress due to reactive oxygen species (ROS). In certain cases, tissue transplants are required, so tissue engineering (TE) proposes possible alternatives for their treatment, such as the application of biological scaffolds (BSs) with antioxidants. This study focused on the evaluation of the antioxidant activity of extracts of Aloysia citrodora (cedrón) and Prunus serotina (capulí) in BSs. Extracts were obtained by maceration and the content of phenols and flavonoids was quantified. By inhibiting DPPH, its antioxidant activity was calculated and a combination of extracts was made. The best combination of extracts was incorporated in the preparation of BSs. To check the antioxidant activity in BS, FT-IR was performed in addition to a DPPH test. Morphological and physical properties were also evaluated by SEM, mechanical tests and permeability. The yield percentages in obtaining extracts were 11.34 for lemon verbena and 12.32 for capulí. The combination of extracts in a 7:3 ratio for capulí:cedron presented an increase in the percentage of inhibition of free radicals compared to pure extracts. The addition of the extract in ABs increased the inhibition percentage to 70.17. Its incorporation into BSs allows the presence of ROS to be reduced. This shows that BS with extract has the potential to be applied in TE for the development of endothelial tissue and combat IHD.