Evaluación de una prueba rápida inmunológica a base de anticuerpos anti-HCG de conejo sobre la detección temprana de preñez en vacas Holstein

Abstract

The aim of this study was to evaluate a rapid immunological test based on rabbit antiHCG antibodies on the early detection of pregnancy in Holstein cows, the present investigation was carried out at the Carmen Ranch located in the community of Hualcanga San Luis, Quero Town, Tungurahua Province. The research was carried out on 70 previously selected cows based on characteristics such as breed (Holstein), age (range 5 - 7 years), weight (range 450 - 500 kg), the same ones that were covered by natural mating. After 15, 30 and 45 days, an immunological test based on rabbit anti-HCG antibodies was applied for early detection of pregnancy in urine samples. In addition, diagnosis by ultrasound was used as a standard or confirmatory test 45 days after the service. The data obtained in the immunological test applied at 15 days showed sensitivity (S) of 76% and specificity (E) of 70%. While the test applied at 30 days indicated S = 86%, E = 85%. Finally, the test applied at 45 days had an S = 92%, E = 90%. The results obtained by applying the immunological test are significant compared to the confirmatory ultrasound test. Based on the results obtained, the use of the ultrasound test is recommended under the conditions of this study.