Uso de plasma autólogo como terapia regenerativa de glándulas mamarias en vacas con mastitis subclínica

Abstract

The objective of this study was to evaluate the use of autologous plasma as regenerative therapy for mammary glands in cows with subclinical mastitis, in the Caserío Pinguili Las Lajas of the Mocha canton. We worked with 20 Holstein cattle of 3 and 6 years (1 to 4 deliveries), these experimental units were divided into 4 groups. The udder health evaluation was carried out by means of the California Mastitis Test (CMT), in order to verify the possible antigenic reaction compatible with mastitis. From the experimental units that were positive in the field test, 4 ml of milk were taken in a test tube and later they were sent to the laboratory to determine the presence of bacterial agents and perform an antibiogram. In addition, in order to obtain blood plasma, blood samples were taken from the jugular vein in test tubes with sodium citrate.The results obtained at the beginning of the trial were: Staphylococcus spp and Staphylococcus aureus, at the end of the experiment we also obtained the presence of Escherichia coli. The treatments were carried out with 5ml, 10ml and 15ml of plasma and with the help of a cannula it was placed on the nipple for three days in a row, resting for ten days and a booster dose on the eleventh day. The data obtained by the Kruskal Wallis test, in the age variable, the mean values obtained were for T1 5.00 a; T2 4.60 a; T3 4.60 a; T4 4.40 a, for the birth variable the means were T1 3.00 n / p; T2 2.60 n / p; T3 2.60 n / p; T4 2.40 n / p and for the colony count the means were T1 32.00 CFU / mL; T2 11.25 CFU / mL; T3 68.89 CFU / mL; T4 58.00 CFU / mL, the results obtained by this test do not show significant differences between treatments. By means of the box plot and the correspondence analysis, it was determined that the results for treatment 2 (5ml of plasma) presented a lower colony count compared to the other treatments, due to the fact that the teats that presented a high colony count Before applying the treatment at the end of the trial, they did not present any type of bacteria.