Evaluación del contenido de Isotiocianatos de los extractos vegetales de mashua (Tropaeolum tuberosum), mostaza (Raphanus raphanistrum L.) y mastuerzo (Tropaeolum majus) y su actividad nematicida in vitro para el control de Meloidogyne

Abstract

The amount of isothiocyanates present in alcoholic and aqueous plant extracts of Mashua (Tropaeolum tuberosum), Mustard (Raphanus raphanistrum L.) and Mastuerzo (Tropaeolum majus) was quantified. The solvents used for extraction were 96 percent ethanol and distilled water. The statistical analysis used was an AXB factorial design. The hydroalcoholic extract of Raphanus raphanistrum L. obtained the highest concentration of isothiocyanate (535,410 ug for ml) and percentage yield (30.67 percent). Same as its in vitro nematicide capacity was assessed against nematodes of the genus Meloidogyne at stage J2 at different concentrations (100, 50, 75, 50, 25, 15, 10, and 5 percent v / v), an absolute control (water distilled) and a chemical control (Metam sodium 1µl for 1L of distilled water) for 48 hours. The concentration with the greatest effect and the shortest time was 100 percent, which reduced the population by 93.33 more less 2.89 percent in the first two hours of exposure and 100 percent mortality at four hours. The assessment of the damage caused to the nematodes was carried out by optical microscopy, observing the internal damages (black-colored bulges) and external damages (detachment of its epidermis). The results obtained in the experimentation were satisfactory, demonstrating that there are new alternatives for the creation of nematicides of organic origin that do not cause damage to the environment and have the same effect as a nematicide of synthetic origin.