“Determinación de cepas y resistencia microbiana en aislamientos bacterianos a partir de úlceras de origen vascular, por presión y secundarias a enfermedades”

Abstract

The objective of the study was to determine strains and microbial resistance in bacterial isolates from ulcers of vascular origin, by pressure and secondary to diseases, the research was based on a qualitative approach, applying field, laboratory, descriptive and correlational research. The protocols and recommendations established for CLSI, were taken into account and applied, and bacterial resistance tests were performed using the Kirby-Bauer disk diffusion method. The sample size comprehends 31 patients (100%) with deep ulcers, who met the inclusion and exclusion criteria, under the rules of asepsis, proceeded with the taking of the evidence in the lesions they presented, with obvious signs of infection, with immediate transport in Stuart medium for processing. Of the 31 biological samples, in 27 crops (87%) there was growth at 24 hours and in 4 crops (13%) there was no growth at 24 and 48 hours. The bacteria were isolated; Staphylococcus aureus with a percentage of 30%, Staphylococcus epidermidis with a percentage of 19%, Pseudomonas aeruginosa with a percentage of 26% and Escherichia coli with a percentage of 26%. It is concluded that the microorganisms with higher incidence were gram-negative bacteria and gram-positive bacteria, no significant differences were observed in the bacterial isolations of the ulcers of vascular origin, by pressure and secondary to diseases.