Fermentación ruminal y síntesis de proteína microbial en ovinos consumiendo dietas a base de Lupinus mutabilis

Abstract

The objective of this research was to determine the effect of the voluntary intake of diets with ascending levels of Lupinus mutabilis on ruminal fermentation, nitrogen balance, microbial protein synthesis in sheep, as well as the effect of diets on the gas production in vitro The work was carried out in the Experimental Teaching Farm Querochaca, located in the sector of the Tambo Cevallos, province of Tungurahua. Five male sheep with an average of 31.3 kg were used, which were given an iso-energetic and iso-protein diet with different levels of Lupinus mutabilis to observe the ruminal function to include tannins in the diet, the animals went through a A fifteen-day adaptation period plus a five-day period where samples were collected for laboratory analysis, resulting in 100-day fieldwork where each animal was confined to its metabolic cage. Voluntary consumption of Condensed Tannins (TC) showed a linear increase (P <0.0001) as Lupinus mutabilis levels were increased in the sheep diet. However, this did not affect the voluntary consumption of nutrients, consumption of PV0.75 and consumption of digestible nutrients, or digestibility of DM, OM and NDF between treatments (P> 0.05). However, PC digestibility was higher (P = 0.0007) in treatments with ascending levels of Lupinus mutabilis in the diet, with a linear and quadratic response (P = 0.0002 and P = 0.0080 respectively). With respect to the digestibility of the FDA, differences (P = 0.0033) between treatments were observed, decreasing the digestibility linearly (P = 0.0003) as TC consumption from Lupinus mutabilis increased in the diet (Table 7). The N-NH3 content in the ruminal fluid was higher (P = 0.0006) in treatments T1 and T2 (29.2 and 27.8 mg / L respectively) with a linear reduction (P = 0.0001) as levels of Lupinus mutabilis in the diet. Regarding volatile fatty acids, there were no differences (P> 0.05) between treatments. However, a linear decrease (P = 0.0065) in acetic acid.